This is the mechanism of peptide cleavage by chymotrpysin. It features the three amino acids shown in the last post. This time I went back to the standard approach for the visualisation. The main feature is that a proton can be passed back and forth between serine and aspartate with histidine in between. This way you always have a proton at hand without the need of a low pH. Chymotrypsin is not something strange that somehow works. It is an amazing nano machine.
I tried to model the steps of the mechanism at first but I do not really know how to do it. Including the whole protein wouldn't work on my laptop. I tried using only the side chains of these three amino acids with fixed Cα positions. It worked out to pass a proton from protonated serine to aspartate. That was pretty nice. But it did not work out well to do more.
Ernest Rutherford: Master of simplicity, mentor extraordinaire - It's Ernest Rutherford's birthday today. Along with Michael Faraday, Rutherford was the greatest experimentalist in modern history and the twentieth centu...
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